PCR-activated cell sorting as a general, cultivation-free method for high-throughput identification and enrichment of virus hosts

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• 作者：Shaun W. Lim^a ; Shea T. Lance^a ; Kenneth M. Stedman^b ; Adam R. Abate^a ; ^{adam@abatelab.org}
• 关键词：Bacteriophage ; Host specificity ; Microfluidics
• 刊名：Journal of Virological Methods
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：14-21
• 全文大小：1549 K
• 卷排序：242

文摘

PACS (PCR-Activated Cell Sorting) can enrich phage-associated bacterial genomes. Ultrahigh-throughput, cultivation-independent, microfluidic technique. Phage digital PCR with in-drop lysis can accurately measure viral titer