Analysis of bacterial community in bulking sludge using culture-dependent and -independent approaches

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• 作者：Decai  ; Jin^a ;   ; ^b ;   ; ^{jindecai19841019@yahoo.com.cn} ; Ping  ; Wang^b ; Zhihui  ; Bai^a ;   ; ^{zhbai@rcees.ac.cn} ; Xinxin  ; Wang^a ;   ; ^b ; Hong  ; Peng^c ; Rong  ; Qi^a ; Zhisheng  ; Yu^b ; Guoqiang  ; Zhuang^a ;   ; ^{gqzhuang@rcees.ac.cn}
• 关键词：bulking sludge ; cultivable bacteria ; rep-PCR ; 16S rRNA gene clone library
• 刊名：Journal of Environmental Sciences
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：23
• 期：11
• 页码：1880-1887
• 全文大小：707 K

文摘

The bacterial community of a bulking sludge from a municipal wastewater treatment plant with anoxic-anaerobic-oxic process was investigated by combination of cultivation and 16S rRNA gene clone library analysis for understanding the causes of bulking. A total of 28 species were obtained from 63 isolates collected from six culture media. The most cultivable species belonged to ¦Ã-Proteobacteria including Klebsiella sp., Pseudomonas sp., Aeromonas sp. and Acinetobacter sp. Further analysis of these strains by repetitive sequence based on polymerase chain reaction (rep-PCR) technology showed that rep-PCR yielded discriminatory banding patterns within the same genus using REP and BOX primer sets. While the culture-independent assessment revealed that ¦Â-Proteobacteria was the dominant group in the bulking sample. Sequence analysis revealed that the highest proportion (14.7 % ) of operational taxonomic units was 98 % similar to Candidatus Accumulibacter phosphatis, which is used to remove phosphorous from wastewater. Our results indicated that combining diff¨¦rent approaches can produce complementary information, thus generate a more accurate view of microbial community in bulking sludge.