Absolute quantification of genetically engineered traits with droplet digital PCR: Effect of DNA treatments and spiking with non-target DNA
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- 作者：Tigst Demekea ; tigst.demeke@grainscanada.gc.ca" class="auth_mail" title="E-mail the corresponding author ; Michelle Holigroskia ; Monika Enga ; Juan Xingb
- 关键词：FP967 flax ; OXY235 canola ; DP305423 soybean ; Low level presence ; Sheared DNA ; ddPCR
- 刊名：Food Control
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：68
- 期：Complete
- 页码：105-111
- 全文大小：1523 K
文摘
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Low levels of GE canola, flax and soybean were analyzed with RainDance ddPCR system.
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Treatment of genomic DNA included non-shearing, QIAshredding and hydroshearing
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Expected results were achieved for non-sheared as well as sheared DNA samples.
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Quantification of 3 GE traits was achieved in non-sheared barley and wheat DNA.
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Overall, there is no need to pre-treat DNA for quantification of GE traits by ddPCR.