Quantitative detection of pork meat by EvaGreen real-time PCR to assess the authenticity of processed meat products
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- 作者：Joana S. Amarala ; b ; Graciete Santosa ; M. Beatriz P.P. Oliveiraa ; Isabel Mafraa ; isabel.mafra@ff.up.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sus scrofa ; Meat products ; Halal products ; Species detection ; Quantification ; Real-time PCR ; Authentication
- 刊名：Food Control
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：72
- 期：part_PA
- 页码：53-61
- 全文大小：775 K
文摘
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Highly sensitive and quantitative detection of pork species.
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Assays of qualitative PCR and real-time PCR with EvaGreen dye were developed.
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Effect of thermal processing on method performance was assessed.
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Normalised quantification of pork meat adulteration in the range of 0.01–10% (w/w).
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Method validation and application to processed Halal meat products.