Bone marrow-derived myofibroblasts promote colon tumorigenesis through the IL-6/JAK2/STAT3 pathway
详细信息 查看全文
- 作者：Liming Zhu ; Xiaojiao Cheng ; Yanfei Ding ; Jindong Shi ; Huanyu Jin ; Hong Wang ; Yunlin Wu ; Jing Ye ; Yiming Lu ; Timothy C. Wang ; Chung S. Yang ; Shui Ping Tu
- 关键词：BMFs ; bone marrow-derived myofibroblasts ; BMF-CM ; BMF conditioned medium ; Co-culture-CM ; co-culture medium of BMFs and cancer cells ; IL-6 ; interleukin-6 ; JAK2 ; janus kinase 2 ; STAT3 ; signal transducer and activator of transcription 3 ; CAFs ; cancer-associated fibroblasts ; MSCs ; mesenchymal stem cells ; EGF ; epithelial growth factor ; bFGF ; basic fibroblast growth factor ; TGF-尾 ; transformation growth factor-尾 ; FL ; firefly luciferase
- 刊名：Cancer Letters
- 出版年：1 February, 2014
- 年：2014
- 卷：343
- 期：1
- 页码：80-89
- 全文大小：2281 K
文摘
Bone marrow-derived myofibroblasts (BMFs) have been shown to promote tumor growth. Here, we found that BMFs or BMF conditioned medium (BMF-CM) induced cancer stem cell-like sphere formation of colon cancer cells. The co-cultured BMFs, but not co-cultured cancer cells, expressed higher levels of IL-6 than BMFs or cancer cells cultured alone. Anti-mouse IL-6 neutralizing antibody, JAK2 inhibitors and STAT3 knockdown in mouse cancer cells reduced BMF- and BMF-CM-induced sphere formation of colon cancer cells. When co-injected, BMFs significantly enhanced tumorigenesis of colon cancer cells in mice. Our results demonstrate that BMFs promote tumorigenesis via the activation of the IL-6/JAK2/STAT3 pathway.