Pork detection in binary meat mixtures and some commercial food products using conventional and real-time PCR techniques
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- 作者：Hassan A. Al-Kahtania ; Elsayed A. Ismaila ; b ; Mohammed Asif Ahmeda ; masifa@ksu.edu.sa" class="auth_mail" title="E-mail the corresponding author
- 关键词：Agarose (PubChem CID ; 11966311) ; Ethidium bromide (PubChem CID ; 14710) ; TAE buffer (PubChem CID ; 21257724)
- 刊名：Food Chemistry
- 出版年：2017
- 出版时间：15 March 2017
- 年：2017
- 卷：219
- 期：Complete
- 页码：54-60
- 全文大小：1776 K
文摘
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PCR assay for species-specific primers was tested (290 bp) for pork DNA.
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Real-time PCR was highly sensitive compared with the conventional PCR for detection of pork.
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Limits of detections (LOD) of pork DNA are useful for the verification of processed meat products.
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Real-time PCR could be an important tool against scandals and illegal meat trade.