Duplex real-time PCR using TaqMan® for the detection of sunflower (Helianthus annuus) and poppy (Papaver rhoeas) in commercial food products
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- 作者：Iné ; s Marí ; a Ló ; pez-Calleja ; ilopezcalleja@vet.ucm.es" class="auth_mail" title="E-mail the corresponding author ; Silvia de la Cruz ; Isabel Gonzá ; lez ; Teresa Garcí ; a ; Rosario Martí ; n
- 关键词：TaqMan real-time PCR ; Sunflower (Helianthus annuus) ; Poppy (Papaver rhoeas) ; Commercial food products ; Food allergy
- 刊名：LWT - Food Science and Technology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：65
- 期：Complete
- 页码：999-1007
- 全文大小：1482 K
文摘
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Duplex real-time PCR-based assay for the simultaneous detection of sunflower and poppy traces in commercial food products.
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This technique allows the sensitive and selective detection of sunflower & poppy with a limit of detection of 0.1 mg kg−1.
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The assay is a useful tool for authenticity control in commercial food products.