Minimal sequence variability of the region of HIV-1 integrase targeted by the Abbott RealTime HIV-1 viral load assay in clinical specimens with reduced susceptibility to raltegravir
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- 作者：Thomas P. Young ; Laura A. Napolitano ; Agnes C. Paquet ; Neil T. Parkin ; Signe Fransen ; Roger Trinh ; Mojgan Haddad ; John Hackett Jr. ; Gavin A. Cloherty
- 关键词：HIV ; Assay ; Integrase inhibitor resistance ; Sequence variability
- 刊名：Journal of Virological Methods
- 出版年：2013
- 出版时间：November, 2013
- 年：2013
- 卷：193
- 期：2
- 页码：693-696
- 全文大小：745 K
文摘
The Abbott RealTime (ART) HIV-1 assay targets the integrase region and is designed to tolerate mismatches. Variability in integrase sequences comprising the assay target regions from >1000 clinical specimens submitted for phenotypic and genotypic raltegravir resistance testing were analyzed. In this large collection of sequences from clinical specimens, the number and location of raltegravir resistance associated mutations did not differ from those tested previously and shown not to result in under-estimation of viral loads.