A 3,3鈥?dichlorobenzidine-imprinted polymer gel surface plasmon resonance sensor based on template-responsive shrinkage
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- 作者：Chunyan Zhou ; Jigang Gao ; Lili Zhang ; Jie Zhou
- 关键词：Molecularly imprinted gel film ; Surface initiated radical polymerization ; Surface plasmon resonance sensor ; 3 ; 3&prime ; -Dichlorobenzidine
- 刊名：Analytica Chimica Acta
- 出版年：17 February, 2014
- 年：2014
- 卷：812
- 期：Complete
- 页码：129-137
- 全文大小：2249 K
文摘
Molecularly imprinted polymer gel film on the gold substrate of a chip was prepared with minute amount of cross-linker for the fabrication of a surface plasmon resonance (SPR) sensor sensitive to 3,3鈥?dichlorobenzidine. The molecularly imprinted gel film was anchored on a gold chip by a surface-bound photo-radical initiator. The sensing of 3,3鈥?dichlorobenzidine is based on responsive shrinkage of the imprinted polymer gel film that is triggered by target binding. This change can improve the responsiveness of the imprinted SPR sensor to 3,3鈥?dichlorobenzidine. The molecularly imprinted polymer gel film was characterized with contact angle measurements, electrochemical impedance spectroscopy, cyclic voltammogram, swelling measurements and atomic force microscopy. The changes of SPR spectroscopy wavenumber shifts revealed that the imprinted gel sensing film can 鈥榤emorize鈥?the binding of 3,3鈥?dichlorobenzidine compared to non-imprinted one. The imprinted gel-SPR sensor showed a linear response in the range of 9.0 脳 10鈭?2 to 5.0 脳 10鈭?0 mol L鈭? (R2 = 0.9998) for the detection of 3,3鈥?dichlorobenzidine, and it also exhibited high selectivity to 3,3鈥?dichlorobenzidine compared to its structurally related analogues. We calculated the detection limits to be 0.471 ng L鈭? for tap water and 0.772 ng kg鈭? for soil based on a signal to noise ratio of 3. The method showed good recoveries and precision for the samples spiked with 3,3鈥?dichlorobenzidine. This suggest that the imprinted gel-SPR sensing method can be used as a promising alternative for the detection of 3,3鈥?dichlorobenzidine.