Specificity and efficacy of noradrenaline, serotonin depletion in discrete brain areas of Swiss mice by neurotoxins
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- 作者：Eric Dailly ; Franck Chenu ; Benoit Petit-Demouliè ; re and Michel Bourin
- 关键词：Noradrenaline ; Serotonin ; Swiss mice ; Depletion
- 刊名：Journal of Neuroscience Methods
- 出版年：2006
- 出版时间：15 January 2006
- 年：2006
- 卷：150
- 期：1
- 页码：111-115
- 全文大小：122 K
文摘
The aim of this work is to define neurotoxins doses to have efficient and specific depletion of noradrenaline (NA), serotonin (5-HT) neurotransmission in cortex, striatum, hippocampus and hypothalamus of Swiss mice after intraperitoneal administration of, respectively, N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP-4) and para-chlorophenylalanine methyl ester hydrochloride (PCPA). The neurotransmitters concentrations were determined by high performance liquid chromatography with amperometric detection. The minimal single dose necessary to produce a highly significant decrease of NA levels (p < 0.01 in comparison with control group) in hypothalamus (−44 % ), hippocampus (−91 % ), striatum (−40 % ) and cortex (−68 % ) was 50 mg/kg but DA and 5-HT levels were modified, respectively, in hypothalamus and striatum. Three doses of PCPA 300 mg/kg over 3 consecutive days involve a profound depletion of 5-HT transmission in all discrete brain areas but NA and DA levels were also significantly reduced. In conclusion, DSP-4 has a different efficacy in discrete brain areas with a noradrenergic specificity which is not absolute, PCPA has a similar efficacy in all brain areas but is unspecific of 5-HT transmission.