Brazilian red propolis improves cutaneous wound healing suppressing inflammation-associated transcription factor NFκB

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• 作者：Flavia Regina Sobreira Corrê ; a^a ; Fernanda Seabra Schanuel^b ; Nathalia Moura-Nunes^a ; André ; a Monte-Alto-Costa^b ; Julio Beltrame Daleprane^a ; ^{juliobd@gmail.com}
• 关键词：BRPE ; Brazilian red propolis extract ; HPLC&ndash ; MS ; high performance liquid chromatography&ndash ; mass spectrometry ; DAB ; diaminobenzidine ; NF- ; κBNF-kappaB ; pNF-κB ; phospho-NF-kappaB ; IL-6 ; interleukin-6 ; TNF-α ; tumor necrosis factor α ; TNF ; tumor necrosis factor ; TGF-β ; transforming growth factor-β ; IL-1 ; interleukin-1 ; PF-4 ; platelet factor 4 ; MCP-1 ; monocyte chemotactic protein-1 ; IFN-γ ; interferon gamma ; CD36 ; thrombospondin receptor ; MMP-9 ; matrix metallopeptidase-9 ; IL-1β ; interleukin-1β
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：86
• 期：Complete
• 页码：162-171
• 全文大小：2591 K
• 卷排序：86

文摘

The use of natural products in wound healing has been extensively studied in the context of complementary and alternative medicine. Propolis, a natural product, is a polyphenol-rich resin used for this purpose. This study aimed to investigate the effect of Brazilian Red Propolis Extract (BRPE) on inflammation and wound healing in mice, using a tissue repair model. The BRPE polyphenol content was analyzed by liquid chromatography coupled to mass spectrometry (LC/MS). A full-thickness excision lesion was created, and mice were treated orally with daily doses of vehicle solution (water-alcohol solution containing 2% of ethanol, control group) or 100 mg/kg of BRPE (P100 group) during nine consecutive days. BRPE chemical composition analysis showed that this complex matrix contains several phenolic compounds such as phenolic acids, phenolic terpenes and flavonoids (especially catechins, flavonols, chalcones, isoflavones, isoflavans, pterocarpans and bioflavonoids). After BRPE administration, it was observed that, when compared to the control group, P100 group presented faster wound closure (p < 0.001); less neutrophils per mm2 (p < 0.05) and macrophages (p < 0.01) in tissue analyses, down regulation of the inflammatory transcription factor pNF-κB protein expression, and reduced production of inflammatory cytokine, such as TGF-β, TNF-α (p < 0.0001), and IL-6 (p < 0.001). These findings suggest a positive role of BRPE oral administration in the wound healing process via suppressing the inflammatory response during tissue repair.