RNAseq profiling of primary microglia and astrocyte cultures in near-term ovine fetus: A glial in vivo-in vitro multi-hit paradigm in large mammalian brain

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• 作者：M. Cortes^a ; ¹ ; M. Cao^b ; ¹ ; H.L. Liu^b ; P. Burns^d ; C. Moore^c ; G. Fecteau^d ; A. Desrochers^d ; L.B. Barreiro^e ; J.P. Antel^c ; M.G. Frasch^a ; ^b ; ^{mfrasch@uw.edu}
• 关键词：Fetus ; Brain ; Microglia ; Astrocytes ; LPS ; in vitro ; in vivo ; Primary culture ; RNAseq
• 刊名：Journal of Neuroscience Methods
• 出版年：2017
• 出版时间：30 January 2017
• 年：2017
• 卷：276
• 期：Complete
• 页码：23-32
• 全文大小：1770 K
• 卷排序：276

文摘

After decades of relative neglect, microglia and astrocytes (also known as glia) are emerging as key players in understanding the brain function and pathophysiology, well beyond their originally conceived “neuronal support” role. Glia shape neuronal development and function in health and disease. In fetal brain, so far methods were limited to study glial function. Here we present a new protocol and paradigm to derive pure primary microglia and astrocytes cultures from a mature fetal sheep brain, a powerful and widely used animal model of human brain development bearing high resemblance to human brain. This technique allows versatile studies of glia function including assessing the genomic properties using next-generation sequencing approaches. With such model we aim to provide a useful novel platform to tackle the challenge of understanding the diversity in glial phenotype and function in a large developing brain.