Bovine hemoglobin: An attractive source of antibacterial peptides

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• 作者：Naï ; ma Nedjar-Arroume ; Vé ; ronique Dubois-Delval ; Estelle Yaba Adje ; Jonathan Traisnel ; Franç ; ois Krier ; Patrice Mary ; Mostafa Kouach ; Gilbert Bri ; Didier Guillochon
• 关键词：Bovine hemoglobin ; Antibacterial peptides ; Pepsin ; Hydrolysate ; Active peptides
• 刊名：Peptides
• 出版年：2008
• 出版时间：June 2008
• 年：2008
• 卷：29
• 期：6
• 页码：969-977
• 全文大小：502 K

文摘

A peptic hemoglobin hydrolysate was fractioned by a semi-preparative reversed-phase HPLC and some fractions have an antibacterial activity against four bacteria strains: Micrococcus luteus A270, Listeria innocua, Escherichia coli and Salmonella enteritidis. These fractions were analyzed by ESI/MS and ESI/MS/MS, in order to characterize the peptides in these fractions. Each fraction contains at least three peptides and some fractions contain five peptides. All these fractions were purified several times by HPLC to obtain pure peptides. Thirty antibacterial peptides were identified. From the isolated antibacterial peptides, 24 peptides were derived from the chains of hemoglobin and 6 peptides were derived from the β chains of hemoglobin. The lowest concentration of these peptides (minimum inhibitory concentration (MIC)) necessary to completely inhibit the growth of four bacteria strain was determined. The cell population of all of the tested bacteria species decreased by at least 97 % after a 24-h incubation with any of the peptides at the minimum inhibitory concentration.