Purification and Partial cDNA Sequence of Acetylcholinesterase from a Korean Strain of the Housefly, Musca domestica

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• 作者：Dae Joong Im ; Won Tae Kim ; Kyung Saeng Boo
• 关键词：acetylcholinesterase ; cloning ; Musca domestica ; ; purification ; resistance
• 刊名：Journal of Asia-Pacific Entomology
• 出版年：2004
• 出版时间：February 2004
• 年：2004
• 卷：7
• 期：1
• 页码：81-87
• 全文大小：514 K

文摘

Acetylcholinesterase (AChE) was purified from adult heads of a Korean housefly, Musca domestica, strain (KNIH) by affinity chromatography on trimethyl (ffj-aminophenyl) ammonium chloride resin. The purified AChE showed the purification factor over 400-fold and the specific activity of about 290 umol/min/mg. The housefly has both the membrane-bound and the soluble forms of AChE. Only the membrane-bound form of the housefly AChE was isolated by Triton X-114 phase partition and revealed on the native gel. The maximum activities of the purified AChE were shown at pH 7.5-8.5 and 40-45°C. Partial cDNA of AChE (795bp) of the KNIH strain showed that its deduced amino acid sequence shared high similarity with that of insecticide-resistant type of insect AChE.