Insecticide Sensitivity of Acetylcholinesterase from a Korean Housefly (Musca domestica

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• 作者：Won Tae Kim ; Kyung Saeng Boo
• 关键词：acetylcholinesterase ; Musca domestica ; Organophosphates ; Purification ; resistance
• 刊名：Journal of Asia-Pacific Entomology
• 出版年：2004
• 出版时间：June 2004
• 年：2004
• 卷：7
• 期：2
• 页码：187-193
• 全文大小：496 K

文摘

Acetylcholinesterase (AChE) was purified, by affinity chromatography, from adult heads of the housefly (Musca domestica) (GSNU strain) known to have mutated AChE gene. The purified AChE showed a single peak in capillary electrophoresis with an overall yield of 42 % representing 957-fold purification and a specific activity of about 290 μ mol/min/mg. It hydrolyzed acetylthiocholine iodide better than S-butyrylthiocholine iodide or propionylthiocholine iodide. AChE-specific inhibitors, BW284C51 and eserine, significantly inhibited the purified AChE, but a butyrylcholinesterase-specific inhibitor, ethopropazine, did not. The housefly AChE was separated into membrane-bound and soluble forms. In vitro inhibition assays with seven organophophates, except for EPN, showed no significant difference between the crude and the purified AChE. Molecular forms of the housefly AChE did not affect the enzyme sensitivity to chlorpyrifos. Inhibition rate ( % ) of the AChE from the housefly was shown lower than that of the susceptible housefly strain (SRS) to trichlorfon and chlorpyrifos possibly because of the mutated amino acids of the AChE gene.