The self-sufficient CYP102 family enzyme, Krac9955, from Ktedonobacter racemifer DSM44963 acts as an alkyl- and alkyloxy-benzoic acid hydroxylase

详细信息    查看全文

• 作者：Natasha K. Maddigan ; Stephen G. Bell ; ^{stephen.bell@adelaide.edu.au}
• 关键词：Cytochrome P450 enzymes ; Monooxygenases ; Fatty acid oxidation ; Ktedonobacter ; Alkylbenzoic acids ; Alkyloxybenzoic acids
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2017
• 出版时间：1 February 2017
• 年：2017
• 卷：615
• 期：Complete
• 页码：15-21
• 全文大小：2142 K
• 卷排序：615

文摘

The heme domain and holoprotein of the self-sufficient Krac9955 enzyme have been purified. The substrate range of Krac9955 has been characterised. A preference for alkyloxy- and alkyl-benzoic acids over fatty acid substrates was observed. The Krac9955 enzyme has a preference for oxidation eight carbons from the carboxylate group. Unusually for a self-sufficient P450 fusion protein the activity of the purified enzyme is low.