A transgenic rat hepatocyte - Kupffer cell co-culture model for evaluation of direct and macrophage-related effect of poly(amidoamine) dendrimers
详细信息 查看全文
- 作者：Katalin Jemnitz<sup>asup><sup> ; sup><sup>jemnitz.katalin@ttk.mta.husup> ; Attila Bá ; tai-Konczos<sup>asup> ; Mó ; nika Szabó ; <sup>asup> ; Enikő Ioja<sup>asup> ; Orsolya Kolacsek<sup>bsup> ; Tamá ; s I. Orbá ; n<sup>bsup> ; Gyö ; rgy Tö ; rö ; k<sup>bsup> ; Lá ; szló ; Homolya<sup>bsup> ; Eszter Ková ; cs<sup>csup> ; Istvá ; n Jablonkai<sup>asup> ; Zsuzsa Veres<sup>asup>
- 关键词：Nanotoxicity ; Poly(amidoamin) dendrimer ; Transgenic rats ; Calcium imaging ; Hepatocyte-Kupffer co-culture
- 刊名：Toxicology in Vitro
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：38
- 期：Complete
- 页码：159-169
- 全文大小：2142 K
- 卷排序：38
文摘
Cells were prepared from rats stably expressing GCaMP2 Ca2 + sensor protein. G5 induced Ca2 + oscillation and sustained Ca2 + signals at 1 and10 μM in hepatocytes. Activation of Kupffer cells by LPS and G5 decreased the inducibility of CYP2B1/2. G5 reduced the canalicular efflux of bilirubin in co-cultures. G5 stimulated the uptake and canalicular efflux of taurocholate.