Application and comparison of high-speed countercurrent chromatography and high-performance liquid chromatography in semi-preparative separation of decarboxymethyl oleuropein aglycone (3,4-DHPEA-EDA), a bioactive secoiridoid from extra-virgin olive oil

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• 作者：Cecilia Jimé ; nez-Sá ; nchez ; Jesú ; s Lozano-Sá ; nchez ; Margret Brü ; ggemann ; Mariana Neves-Vieira ; Miriam Rodriguez-Werner ; Eva Schmalfuß ; Peter Winterhalter ; Antonio Segura-Carretero and Alberto Ferná ; ndez-Gutié ; rrez
• 刊名：European Journal of Lipid Science and Technology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：119
• 期：2
• 全文大小：738K
• ISSN：1438-9312

文摘

This paper primarily extends research on applying and comparing high-speed countercurrent chromatography (HSCCC) and high-performance liquid chromatography (HPLC) in semi-preparative isolation of decarboxymethyl oleuropein aglycone (3,4-DHPEA-EDA), a bioactive secoiridoid, from extra-virgin olive oil (EVOO). An EVOO phenolic extract (EVOO-PE) was obtained by solid-phase extraction (SPE), and this extract was subjected to one run using the maximum loading capacity of each methodology without loss of resolution. For this sample set, the HSCCC method proved to be a more efficient approach regarding loading capacity, solvent consumption, and throughput compared with the HPLC method. However, the purity of the target from HPLC was superior to that of HSCCC, as the number of theoretical plates could not match that of HPLC. Therefore, we propose that HPLC should be used when higher purities are required, despite higher costs. In addition, HPLC is orthogonal and complementary to HSCCC, and can often provide straightforward resolution of otherwise difficult separations by HSCCC.