Methylenetetrahydrofolate Reductase CpG Islands: Epigenotyping
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- 作者：Loo Keat Wei ; Heidi Sutherland ; Anthony Au ; Emily Camilleri ; Larisa M Haupt ; Siew Hua Gan and Lyn R Griffiths
- 刊名：Journal of Clinical Laboratory Analysis
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：30
- 期：4
- 页码：335-344
- 全文大小：15028K
- ISSN：1098-2825
文摘
Determination of the differential DNA methylation patterns of methylenetetrahydrofolate reductase (MTHFR) that are associated with differential MTHFR activity is important to understand the pathogenesis of ischemic stroke. However, to date, no data are available on the differential DNA methylation profiles of Kelantanese Malays. Therefore, we developed a rapid and efficient serial pyrosequencing assay to determine differential DNA methylation profiles of MTHFR, which help to further our understanding of the pathogenesis of ischemic stroke. The developed assay also served as the validation platform for our previous computational epigenetic research on MTHFR.