2-Substituted dATP Derivatives as Building Blocks for Polymerase-Catalyzed Synthesis of DNA Modified in the Minor Groove

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• 作者：Já ; n Matyašovský ; Dr. Pavla Perl&iacute ; ková ; Dr. Vincent Malnuit ; Dr. Radek Pohl and Prof. Dr. Michal Hocek
• 刊名：Angewandte Chemie
• 出版年：2016
• 出版时间：December 19, 2016
• 年：2016
• 卷：128
• 期：51
• 页码：16088-16091
• 全文大小：1694K
• ISSN：1521-3757

文摘

2′-Deoxyadenosine triphosphate (dATP) derivatives bearing diverse substituents (Cl, NH₂, CH₃, vinyl, ethynyl, and phenyl) at position 2 were prepared and tested as substrates for DNA polymerases. The 2-phenyl-dATP was not a substrate for DNA polymerases, but the dATPs bearing smaller substituents were good substrates in primer-extension experiments, producing DNA substituted in the minor groove. The vinyl-modified DNA was applied in thiol–ene addition and the ethynyl-modified DNA was applied in a CuAAC click reaction to form DNA labelled with fluorescent dyes in the minor groove