Towards electroenzymatic processes involving old yellow enzymes and mediated cofactor regeneration

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• 作者：Andreas Tosstorff ; Cora Kroner ; Diederik J. Opperman ; Frank Hollmann and Dirk Holtmann
• 刊名：Engineering in Life Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：17
• 期：1
• 页码：71-76
• 全文大小：593K
• ISSN：1618-2863

文摘

Old yellow enzymes are able to catalyze asymmetric C=C reductions. A mediated electroenzymatic process to regenerate the NADPH in combination with an old yellow enzyme was investigated. Due to the fact that the overall process was affected by a broad set of parameters, a design of experiments (DoE) approach was chosen to identify suitable process conditions. Process conditions with high productivities of up to 2.27 mM/h in combination with approximately 90% electron transfer efficiency were identified.