蒙古扁桃药材正丁醇提取物降血脂作用的量效关系及其化学成分的研究
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摘要
目的:探索蒙古扁桃正丁醇提取物降血脂作用的量效关系,研究其化学成分。方法:使用正丁醇提取蒙古扁桃种仁,得正丁醇提取物。大鼠按体重均衡随机分组,分别为空白组和模型组,空白组给予普通饲料饲养,模型组采用高脂饲料喂养法建立高脂模型。建模成功后,持续按前述方法饲养,将大鼠分为空白组、模型对照组、阳性药组(辛伐他汀)、蒙古扁桃药材正丁醇提取物给药组。空白组与模型对照组灌胃给予生理盐水,实验组以不同剂量的蒙古扁桃正丁醇提取物(0.25、0.5、1.0、2.0、4.0 g/kg)灌胃。给药七周后测定大鼠血清中Total cholesterol(TC)、Triglyceride(TG)、High-density lipoprotein cholesterol(HDL-C)、Low-density lipoprotein cholesterol(LDL-C)的含量;使用色谱、波谱手段分离提纯蒙古扁桃正丁醇提取物。结果:与高脂模型组相比,0.25、0.5、4.0 g/kg正丁醇提取物无显著降血脂作用,1.0、2.0 g/kg正丁醇提取物组能显著降低TC,LDL-C含量(p<0.05),且以2.0 g/kg的效果最好;从蒙古扁桃正丁醇提取物中提取出来的化合物为苦杏仁苷单体。结论:蒙古扁桃药材正丁醇提取物起降血脂的剂量在1.0~2.0 g/kg之间,且以2.0 g/kg生药量最好;苦杏仁苷为首次从蒙古扁桃种仁中分离出来。
Objective: To explore the dose-effect relationship of n-butanol extracts of Amygdalus mongolica on reducing blood lipid and study the chemical constituents of the n-butanol extracts of Amygdalus mongolica. Methods: Kernels of Amygdalus Mongolica was extracted by n-butanol to get the n-butanol extracts. Wistar rats were randomly assigned into two weight balanced groups: the blank control group and the model group. The blank control group was fed with general diets,the model group was fed with high fat diets to build hyperlipemia model.After the models were successfully built,keeping in accordance with the above feeding methods,Wistar rats were divided into four treatment groups: the blank control group,the model group,the positive drug group( Simvastatin) and the different doses of n-butanol extracts treatment group. The blank control and the model group were treated with physiological saline by intragastric administration,the different doses of n-butanol extracts treatment group were treated with 0.25,0.5,1.0,2.0,4.0 g / kg n-butanol extracts by intragastric administration respectively.After seven weeks of administration,the contents of total cholesterol( TC),triglyceride( TG),high-density lipoprotein cholesterol( HDL-C) and low-density lipoprotein cholesterol( LDL-C) were determined. N-butanol extracts of Amygdalus mongolica were separated and purified by chromatographic and spectroscopic methods. Results: Comparing to hyperlipemia model,0.25,0.5 g / kg and 4.0 g / kg n-butanol extracts didn't show significant effect of reducing blood lipid,while 1.0 g / kg and 2.0 g / kg n-butanol extracts could significantly reduce the contents of TC and LDL-C( p < 0.05),and the effect of2.0 g / kg n-butanol extracts was the best; amygdalin was isolated from n-butanol extracts of Amygdalus mongolica.Conclusion:The dose of n-Bu OH extracts being effective ranged from 1.0 g / kg to 2.0 g / kg,and the dose of 2.0 g / kg n-Bu OH extractsshowed best effect.Amygdalin was isolated from kernels of Amygdalus mongolica for the first time.
Objective: To explore the dose-effect relationship of n-butanol extracts of Amygdalus mongolica on reducing blood lipid and study the chemical constituents of the n-butanol extracts of Amygdalus mongolica. Methods: Kernels of Amygdalus Mongolica was extracted by n-butanol to get the n-butanol extracts. Wistar rats were randomly assigned into two weight balanced groups: the blank control group and the model group. The blank control group was fed with general diets,the model group was fed with high fat diets to build hyperlipemia model.After the models were successfully built,keeping in accordance with the above feeding methods,Wistar rats were divided into four treatment groups: the blank control group,the model group,the positive drug group( Simvastatin) and the different doses of n-butanol extracts treatment group. The blank control and the model group were treated with physiological saline by intragastric administration,the different doses of n-butanol extracts treatment group were treated with 0.25,0.5,1.0,2.0,4.0 g / kg n-butanol extracts by intragastric administration respectively.After seven weeks of administration,the contents of total cholesterol( TC),triglyceride( TG),high-density lipoprotein cholesterol( HDL-C) and low-density lipoprotein cholesterol( LDL-C) were determined. N-butanol extracts of Amygdalus mongolica were separated and purified by chromatographic and spectroscopic methods. Results: Comparing to hyperlipemia model,0.25,0.5 g / kg and 4.0 g / kg n-butanol extracts didn't show significant effect of reducing blood lipid,while 1.0 g / kg and 2.0 g / kg n-butanol extracts could significantly reduce the contents of TC and LDL-C( p < 0.05),and the effect of2.0 g / kg n-butanol extracts was the best; amygdalin was isolated from n-butanol extracts of Amygdalus mongolica.Conclusion:The dose of n-Bu OH extracts being effective ranged from 1.0 g / kg to 2.0 g / kg,and the dose of 2.0 g / kg n-Bu OH extractsshowed best effect.Amygdalin was isolated from kernels of Amygdalus mongolica for the first time.
引文
[1]范丽丽,龚一萍.辨证治疗高脂血症的研究进展[J].中华中医药学刊,2007,25(11):2333-2335.[2]Basciano H,FEDERICOL L.Fructose,insulin resistance and metabolic dyslipidemia[J].Nutrition Metab,2005,2(1):1-14.[3]Maria A.The reduction of dietary sucrose improves dyslipidemia,adiposity and insulin secretion in an insulin resistant rat model[J].Cnin Trad it Herb Drugs,2001,32(9):8522-8551.[4]冯利民,李立凤,杜武勋,等.高脂血症基本病机与证型规律研究进展[J].时珍国医国药,2012,23(12):3101-3103.[5]袁娟,卫娜,徐勇,等.白豆中提取的α-淀粉酶抑制剂调节高脂血症的研究[J].食品工业科技,2014,35(23):127-129,135.[6]王飞.辛伐他汀的临床不良反应研究[J].中国现代药用,2009,3(1):107-108.[7]周红军,郭巍.中药治疗高血脂症的临床研究进展[J].现代中西医结合杂志,2011,20(20):2592-2593.[8]马毓泉.内蒙古植物志(第三卷)[M].呼和浩特:内蒙古人民出版社,1989:519-522.[9]杜巧珍,红雨,包贺喜图.珍稀濒危植物蒙古扁桃研究进展[J].内蒙古师范大学学报:自然科学汉文版,2010,39(3):309-313.[10]白迎春,石松利,钮树芳,等.蒙古扁桃药材对大鼠高脂血症的影响[J].时珍国医国药,2012,23(10):2406-2407.[11]吴培赛,石松利,周红兵,等.蒙古扁桃药材不同提取物对高脂血症大鼠血脂和肝功能的影响[J].中国实验方剂学志,2015,21(21):113-117.[12]陈晓燕.桃仁、桑叶化学成分及生物活性研究[D].北京协和医学院,2014.[13]姬凤彩,姚刚,李琳琳,等.对大鼠高脂血症模型分型的讨论[J].新疆医科大学学报,2007,30(11):1297-1298.[14]段金廒,范欣生,宿树兰,等.中药及方剂量效关系的研究进展与思考[J].南京中医药大学学报,2009,25(4):241-245.[15]谢晚晴,连凤梅,姬航宇,等.中药量效关系研究进展[J].中医杂志,2011,52(19):1696-1699.[16]王彬辉,章文红,张晓芬,等.苦杏仁苷提取工艺及药理作用研究新进展[J].中华中医药学刊,2014,32(2):381-384.[17]DENG J,LI C,WANG H,et al.Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells[J].Biochem Biophys Res Commun,2011,411(3):523-529.