The stable isotopic information in biogenic calcite was used to calculate the isotopic composition of seawater in the geological history. Brachiopod shell, due to its resistant ability to diagenesis (low magnesium calcite) and widespread distribution, was considered to be one of the ideal samples in the study of seawater isotopic composition in Paleozoic. The authors measured carbon and oxygen isotopic composition of well-preserved Longmenshan brachiopod shells, and established the seawater isotopic variation curve of Devonian and discussed the influencing factors. The results shows that: the δ