In order to assess the influence of seafloor hydrothermal activity on element occurrence in sediments, the authors carried out sequential extraction procedures and whole-rock analysis for the surface sediments (17A-EPR-TVG1, 17A-EPR-TVG5) collected from East Pacific Rise near 13°N and the equator, and discussed mode of element occurrence of Al, Ca, Fe, Mn, Ti, Ba, Zn, Cu, Sr, V, Mo, U and rare earth elements (REE). XRD results indicate that the primary mineral phase of 17A-EPR-TVG1 is pyrite and 17A-EPR-TVG5 sample has calcite as its main mineral. Elements except Ca and Sr of 17A-EPR-TVG1 which were greatly affected by hydrothermal activity mainly occur in the residual phase and ferromanganese oxide phase.