///Comparable studies were conducted between chemical oxidation and microbial oxidation of ferrous sulfate by a mixed culture of Acidithiobacillus ferrooxidans in the presence of different concentrations of As(Ⅴ). Eh, pH and Fe2+ concentration were monitored periodically and the final precipitation was analyzed by X-ray diffraction (XRD) and SEM. The data obtained showed and chemical oxidation of Fe2+ was very slow with a final oxidizing ratio of <8% and As(Ⅴ) had no effect on Fe2+ oxidation chemically. Slight enhancement of Fe2+ oxidation was observed by 100 mg/ L As(Ⅴ) in the microbial system. Complete oxidation of Fe2+ could be reached in about 60 hours in the presence of A. ferrooxidans with 500 mg~