Enrichment and Cultivation of Natural Methanogen and Simulation of Biogenetic Gas Generation from Brown Coal Samples

• 责任者：Wang Aikuan (Key Laboratory of CBM Resources and Pooling Process ; Ministry of Education of China ; Xuzhou 221116 ; China) ; Qin Yong
• 刊名：Geological Journal of China Universities
• 出版年：2010
• 卷期：16(1)
• 关键词：生物气 ; biogenetic gas ; 煤层气 ; coalbed gas
• 页码：p.80-85
• 图表：2 illus., 3 tables, 25 refs.
• 分类号：1003
• issnNo：ISSN1006-7493
• isbnNo：CN32-1440/P

Using the method of anaerobic glove chamber culture, active anaerobic bacteria were successfully enriched and cultivated in brown coal samples collected from Zhaotong Basin, Yunnan Province. Simulation experiments of biogenic gas for 60 days were further conducted to analyze the generation regularities, substance compositions and genetic mechanism of biogenic gas. The results showed that in all three brown coal samples there existed active anaerobic bacteria, among which cellulose decomposition bacteria were in majority and sulfate-reducing bacteria were very few, which means that the biochemical circumstances in the brown coal-seams of Zhaotong Basin were reductive and favorable for the growth of methanogen.