Advances in the Study of the Denitrifier Method for the Measurement of Isotopic Compositions of Nitrate

• 责任者：Zhang Cuiyun (Institute of Hydrogeology & Environmental Geology ; Chinese Academy of Geology Sciences ; Shijiazhuang 050061 ; China) ; Zhang Junxia
• 刊名：Advances in Earth Science
• 出版年：2010
• 卷期：25(4)
• 关键词：细菌 ; bacteria ; 反硝化作用 ; denitrification ; 同位素分析 ; isotopic analysis
• 页码：p.360-364
• 图表：24 refs.
• 分类号：0900
• issnNo：ISSN1001-8166
• isbnNo：CN62-1091/P

In the 21st century, the significant advances in the measurement of nitrogen and oxygen isotopic compositions of nitrate are the development of analytical techniques of the denitrifier method. The experimental procedure consists of several key steps including bacterium selection and cultivation, conversion of sample NO₃^- to N₂O, extraction and isotopic analysis of N₂O and correction of isotopic analytical results. Results from foreign researchers showed that the technique was applicable for the measurement from soil, seawater and freshwater, and was widely used. This technique was also developed in and obtained important advances in the contamination study of nitrate in the vadose zone and groundwater.